High-Performance Thin-Layer Chromatography Method Development for Estimation of Triterpenoids in Calotropis Gigantea Roots
نویسندگان
چکیده
Development of analytical method on high performance thin layer chromatography for estimation content marker compound, preferably bioactive constituent, in traditional herb has become a rational approach its standardization. The literature does not reveal any report phytochemical investigation to standardize Calotropis gigantea roots the basis compounds. Non-availability such study prompted us develop triterpenoids (taraxerol and lupeol), which are compounds plant. Numerous solvent systems were tried resolve these methanol extract but best resolution taraxerol lupeol was obtained using toluene:ethyl acetate:methanol (8:1:1) toluene:chloroform (3:7), respectively, as systems. developed plates derivatized by treating with anisaldehyde-sulphuric acid reagent followed heating. visible coloured bands scanned at 550 nm. percentage 0.0086 % 0.0093 w/w, roots. consistent reproducible therapeutic effects herbal preparations containing can be achieved if based standardized raw material plant is used.
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ژورنال
عنوان ژورنال: Indian Journal of Pharmaceutical Sciences
سال: 2022
ISSN: ['0250-474X', '1998-3743']
DOI: https://doi.org/10.36468/pharmaceutical-sciences.1059